BioXplorer 400P | High-pressure bench-top, parallel 4 bioreactor platform

The BioXplorer 400P is an automated parallel pressure bioreactor system for the discovery and optimization of novel bioprocesses. Designed for applications ranging from syngas fermentation to cell-free enzymatically catalyzed processes, the BioXplorer 400P uses pressure and precision gas control to accelerate bioprocesses, which depend on the availability of dissolved gases.

The customizable configuration combined with versatile software allows the BioXplorer 400P to fulfill your precise needs, giving you the data you need to scale up faster.

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Biotechnology Solutions

BioXplorer Specification Book

Overview

Pressurized Bioprocessing

The BioXplorer 400P’s ability to run up to four parallel bioprocesses at up to 10 bar pressure is unique. More and more bioprocess scientists are turning to pressurized systems to increase the availability of dissolved gases and accelerate gas-dependent bioprocesses. The BioXplorer 400P is an indispensable tool for the optimization and scale-up of such processes. The system has been engineered with a focus on safety and ease of use. For example, the use of 2 gas spargers per reactor enables risks associated with potentially hazardous gas mixtures to be minimized. Adding pressure to accelerate your bioprocesses can deliver higher yields without compromising functionality and configurability.

Configurable System

The BioXplorer 400P uses a modular design and can be supplied with a wide range of probes, gas feeds, and liquid feeds, enabling it to be tailored to your precise needs. This maximizes functionality and ease of use while minimizing bench space requirements and cost.

Flexibility to optimize processes

The choice of probes to track the wide range of physicochemical parameters in bioprocess provides in-depth knowledge. Using H.E.L’s WinISO tight control and responsiveness, automatization of the process is possible. BioXplorer 400P can operate 4 bioreactors in parallel or independently, reducing the optimization time of bioprocesses.

Features and Options

Specification Point	BioXplorer 400P
Number of independent parallel bioreactors	4
Bioreactor material	Stainless steel body and lid
Total volume	500ml
Working volume	120 – 400 ml
Pressure rating	Up to 10 bar
Sterilization	Autoclavable bioreactors (including sensors)
Temperature Range	0 – 135^°C
Temperature resolution	0.1^°C
Stirrer drive	Magnetically driven captive impeller or Overhead agitation (magnetically coupled)
Stirrer speed	Magnetically Driven – 250 – 1500 rpm Overhead Agitation – 20- 2000rpm
Auto re-couple	Magnetically Driven – Yes Overhead Agitation – N/A
Liquid additions with independent control for each bioreactor	Magnetically Driven – 2 as standard, options for up to 2 additional liquid feeds Overhead Agitation – 2 as standard, option for 1 additional liquid feed
Gas additions	2 sets of 4 mass flow controllers allowing independent control for each reactor as standard, option for 2 additional sets
Standard options for MFC calibration	Air, oxygen, nitrogen, hydrogen, carbon monoxide, carbon dioxide or methane
Gas spargers	2
Exhaust gas	Peltier cooled condenser
pH control	1-way control (2-way control optional)
pH Sensing	0-14
Polarographic DO sensing	Optional
DO control	Gas/liquid flow rate, stirrer speed (Optional)
BioVIS – Understand cell density trends without taking samples	Optional
Foam/Level detection	Optional
Control PC and software	Windows® PC with WinISO control software preinstalled: Fully independent control of all reactors Advanced control strategies for stirring and liquid and gas additions based on feedback from sensors, time-based profiles, or event detection Real-time display and logging of all reaction parameters Easy export of data in a range of formats, including .csv

*The upper temperature limit for data acquisition from standard DO probes is 40 ˚C. Speak to one of our specialists to discuss alternative DO probes for use at elevated temperatures. The lower temperature limit requires integrated circulator option.