BioXplorer 5000 | Bioreactor With High-Pressure

The BioXplorer 5000 bioreactor is ideally suited to drive your cell culture scale-up efficiency while staying on the bench. With culture volumes between 500 ml and 20 L, the BioXplorer 5000 provides fully automated control of your cell culture.

The systems are available either in standard configurations or can be customized to meet your requirements. With our open system architecture and flexible software offering simple upgrade and integration options.

Increase your process knowledge and take advantage of an exhaustive list of standard options and innovative features including automated inventory monitoring, online cell density analysis, and automatic KLa determination. You can also add specific gas feeds to run cultures at elevated pressures, under aerobic or anaerobic conditions, ideal for C1 gas studies.

The H.E.L BioXplorer 5000 bioreactor is suitable for a wide range of applications including microbial and mammalian cell cultures with:

  • Automated inventory monitoring
  • Online cell density analysis
  • Choice of reactor sizes and types, including elevated pressure designs (5 bar or 10 bar options)
  • Fully automated control of multiple parallel reactors

 

Software

The H.E.L control software WinISO runs the BioXplorer 5000 system, enabling complex, fully automated experiments to run unattended.
This enables:

  • Multi reactor control with unlimited step recipe planning. Plans can be changed online at any time without process interruption
  • Starting and stopping reactors individually or simultaneously
  • The individual reactor temperature and agitation control
  • User-defined data-logging with customisable tabular and graphical displays
  • Export data functions and integration with in-house electronic lab notebooks and record-keeping systems
  • DoE and QbD package integration

More specifically, the software controls a wide range of system features, including:

  • Temperature
  • Gas feeds, flow and mix
  • pH
  • Dissolved oxygen
  • Redox
  • Stirrer speed
  • Torque (for viscosity indication)
  • Pressure
  • Liquid feed rates
  • Antifoam and level detection

Gas Feeds

  • Choose from simple or more sophisticated gas flow control regimes
  • Each bioreactor can be configured with single or multiple mass flow controllers
  • For microbial fermentations, automatic oxygen enrichment is possible
  • For cell culture applications CO2 can be used for pH control
  • Ratios of several gases can be mixed and regulated with online feedback from off-gas analytics

 

Liquid Feeds

  • Choice of liquid feeds for standard and pressure applications
  • Highly linear peristaltic pump drives are integrated into standard heads which enable exceptional pH and dosing control as well as a wide operating range
  • Syringe pumps and high-pressure pumps are also available

pH, Dissolved Oxygen and Redox

  • A wide range of  probes are available to cater for different culture types, reactor sizes and applications
  • All probes are integrated into the WinISO software to allow automated monitoring and feedback loops

 

Off Gas Analysis

  • Tandem off-gas analysis units monitor and control a culture process continuously
  • This enables automatic calculation of oxygen uptake rate (OUR);  carbon dioxide production rate (CPR) and respiration quotient (RQ)

 

Cell Density and Biomass Monitoring

  • BioVIS enables the online monitoring of cell growth and biomass using optical density, removing  the need for online sampling
  • Track cell growth and understand the processes without taking samples
  • Viable cell density probes can also be integrated

 

Download our BioXplorer 5000 brochure for more details

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