BioXplorer 5000 | Lab-scale bioreactor platform

The BioXplorer 5000 bioreactor is ideally suited to drive your cell culture scale-up efficiency while staying on the bench. With culture volumes between 500 ml and 20 L, the BioXplorer 5000 provides fully automated control of your cell culture.

The systems are available either in standard configurations or can be customized to meet your requirements. With our open system architecture and flexible software offering simple upgrade and integration options.

The BioXplorer 5000 range is ideal for:

Microbial and mammalian cell culture and gas fermentation
Elevated (high) pressure design optional
Process development and optimization and scale-up studies

Increase your process knowledge and take advantage of an exhaustive list of standard options and innovative features including automated inventory monitoring, online cell density analysis, and automatic KLa determination. You can also add specific gas feeds to run cultures at elevated pressures, under aerobic or anaerobic conditions, ideal for C1 gas studies.

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Features

The H.E.L BioXplorer 5000 bioreactor is suitable for a wide range of applications including microbial and mammalian cell cultures with:

Automated inventory monitoring
Online cell density analysis
Choice of reactor sizes and types, including elevated pressure designs (5 bar or 10 bar options)
Fully automated control of multiple parallel reactors

Software

The H.E.L control software runs the BioXplorer 5000 system, enabling complex, fully automated experiments to run unattended.
This enables:

Multi reactor control with unlimited step recipe planning. Plans can be changed online at any time without process interruption
Starting and stopping reactors individually or simultaneously
The individual reactor temperature and agitation control
User-defined data-logging with customisable tabular and graphical displays
Export data functions and integration with in-house electronic lab notebooks and record-keeping systems
DoE and QbD package integration

More specifically, the software controls a wide range of system features, including:

Temperature
Gas feeds, flow and mix
pH
Dissolved oxygen
Redox
Stirrer speed
Torque (for viscosity indication)
Pressure
Liquid feed rates
Antifoam and level detection

Options

Gas Feeds

Choose from simple or more sophisticated gas flow control regimes
Each bioreactor can be configured with single or multiple mass flow controllers
For microbial fermentations, automatic oxygen enrichment is possible
For cell culture applications CO2 can be used for pH control
Ratios of several gases can be mixed and regulated with online feedback from off-gas analytics

Liquid Feeds

Choice of liquid feeds for standard and pressure applications
Highly linear peristaltic pump drives are integrated into standard heads which enable exceptional pH and dosing control as well as a wide operating range
Syringe pumps and high-pressure pumps are also available

pH, Dissolved Oxygen and Redox

A wide range of probes are available to cater for different culture types, reactor sizes and applications
All probes are integrated into the WinISO software to allow automated monitoring and feedback loops

Off Gas Analysis

Tandem off-gas analysis units monitor and control a culture process continuously
This enables automatic calculation of oxygen uptake rate (OUR); carbon dioxide production rate (CPR) and respiration quotient (RQ)

Cell Density and Biomass Monitoring

BioVIS enables the online monitoring of cell growth and biomass using optical density, removing the need for online sampling
Track cell growth and understand the processes without taking samples
Viable cell density probes can also be integrated

Technical Literature

The following is a list of supporting Technical Literature.

The use of a H.E.L BioXplorer in fermentations of Cupriavidus necator H16 at elevated pressure using CO2 as the sole carbon source

Publications

The following are a list of some technical publications which highlight the use of the equipment.

Isobutanol production freed from biological limits using synthetic biochemistry

S. Sherkhanov, T.P. Korman, S.Chan, S. Faham, H. Liu, M.R. Sawaya, WT. Hsu, E. Vikram, T. Cheng, J.U. Bowie

01-Dec-2020

https://doi.org/10.1038/s41467-020-18124-1(Subscription or purchase maybe required for full access)

BioXplorer 5000 | Lab-scale bioreactor platform