Bioreactor platformsConfigured for your bioprocess

BioXplorer Parallel, High-Pressure and Scale-Up Bioreactors

Q: Which BioXplorer is right for my process?

Start from throughput and scale: the BioXplorer 100 gives eight reactors at 50–150 ml — or sixteen on the 100XL — for maximum screening throughput; the 400 gives four at 120–400 ml, or eight on the 400XL. For gas-dependent work under pressure, the 100P and 400P run the same two configurations in stainless steel at up to 10 bar; for scale-up, the 5000 is a single 1–5 L bioreactor — glass at ambient pressure, or stainless steel at up to 10 bar (5000P). A specialist can help you match the platform to your application.

Q: Is the system customisable to my process?

Yes. Every BioXplorer is configured to your requirements — vessels, probes, sensors, gas and liquid feeds are all specified around your application. Customisation to the customer’s process is central to H.E.L’s approach.

Q: What working volumes are available?

From 50 ml to 5 L across the family: 50–150 ml (100 and 100XL), 120–400 ml (400, 400XL and the P variants) and 1–5 L interchangeable vessels (5000) — so methods developed at screening scale carry through to scale-up.

Q: Can I run gas-dependent bioprocesses under pressure?

Yes. The BioXplorer 100P and 400P run stainless-steel reactors (eight or four) at up to 10 bar with dual gas spargers per reactor and MFC calibrations for air, O₂, N₂, H₂, CO, CO₂ and methane; the 5000P scales the same capability to 5 L. Typical applications include syngas fermentation, CO₂ biomanufacturing and Power-to-Liquid.

Q: What does the control software do?

The H.E.L WinISO software runs every reactor with fully independent, automated control — advanced strategies from sensor feedback, time-based profiles or event detection, with unlimited step-recipe planning, real-time logging, .csv export and ELN integration.

Q: What monitoring and analytics options are available?

pH and dissolved-oxygen sensing as standard; optional BioVIS for online cell-density monitoring without sampling, foam/level detection, and Tandem off-gas analysis for OUR, CPR and RQ. The 5000 also integrates FTIR, particle-sizing and Raman.

Q: Are the bioreactors autoclavable?

Yes — the parallel platforms use autoclavable bioreactors, including sensors, with glass bodies and stainless-steel lids (fully stainless on the P variants).

Five bioreactor platforms, one system — screen in parallel, pressurise to 10 bar, then scale to 5 litres, all under the same WinISO control.

Ask a specialist Download specifications book Biotechnology brochure

What do you need to do?

1Screen strains & media in parallelBioXplorer 100 · 400 2Run fermentations at up to 10 barBioXplorer 100P · 400P 3Scale up to 5 litresBioXplorer 5000 · 5000P

Not sure which? Compare the range side by side ↓

Parallel screening

The BioXplorer 100 & 400 series — one bench-top platform with 4 to 16 independent bioreactors, 50–400 ml, for high-throughput bioprocess screening and optimisation.

High-pressure parallel screening

BioXplorer 100P & 400P — the parallel platform in stainless steel at up to 10 bar, with 8 or 4 bioreactors, for gas-dependent bioprocesses from syngas fermentation to cell-free biocatalysis.

Scale-up & custom

BioXplorer 5000 & 5000P — a single, highly automated bioreactor, 1–5 L, in glass at ambient pressure or stainless steel at up to 10 bar.

The BioXplorer 100 & 400 series — parallel screening on the bench

One bench-top platform in four configurations: 8 or 16 bioreactors at 50–150 ml for maximum experimental throughput, and 4 or 8 at 120–400 ml where working volume matters. Control, monitoring, software and options are the same on all four.

8 or 16 reactors · 50–150 ml

BioXplorer 100 and 100XL glass bioreactors

BioXplorer 100 / 100XL

Independent bioreactors for high-throughput screening and DoE at the smallest practical volume — modest working volumes cut media and reagent use. Eight reactors on the 100, doubled to sixteen on the 100XL for the highest screening throughput on the platform. Magnetically driven captive impeller, 250–1500 rpm.

4 or 8 reactors · 120–400 ml

BioXplorer 400 and 400XL glass bioreactors

BioXplorer 400 / 400XL

Independent bioreactors where working volume matters — four on the 400, doubled to eight on the 400XL for the fast optimisation of critical bioprocess parameters. Choice of magnetic stirring (250–1500 rpm) or overhead agitation (20–2000 rpm) for more demanding, viscous or high-density cultures.

Shared across both configurations

Independent pH, DO, temperature and feeding in every bioreactor
Glass body, stainless steel lid — autoclavable bioreactors, including sensors
0 – 135 °C with 0.1 °C temperature resolution
2 liquid feeds per reactor as standard, options for up to 2 additional feeds
1 set of mass flow controllers giving independent gas control per reactor, option for 1 additional set
pH sensing 0–14 with 1-way control (2-way optional); polarographic DO sensing as standard
Peltier cooled exhaust condenser
WinISO control software pre-installed — automated control from sensor feedback, real-time logging and .csv export
Optional BioVIS cell-density monitoring, Tandem off-gas analysis and foam/level detection

Every BioXplorer is configured to your requirements — probes, sensors, gas and liquid feeds all specified around your process.

CO₂ fermentationSustainable aviation fuel Industrial biotechnologyAerobic fermentation High-density cultivationYeast fermentation Gas fermentationSynthetic biology Food biotechnologyWaste-gas fermentation

Glass bioreactors for the BioXplorer 100, 400 and XL — The glass reactor family — 100, 400 and XL

Glass bioreactor with liquid feeds and monitoring — Autoclavable vessel with feeds and monitoring

Specifications

Specification	BioXplorer 100 / 100XL	BioXplorer 400 / 400XL
Number of independent parallel bioreactors	8 (100) · 16 (100XL)	4 (400) · 8 (400XL)
Total volume	200 ml	500 ml
Working volume	50 – 150 ml	120 – 400 ml
Bioreactor material	Glass body, stainless steel lid
Sterilization	Autoclavable bioreactors (including sensors)
Temperature range	0 – 135 °C
Temperature resolution	0.1 °C
Stirrer drive	Magnetically driven captive impeller	Magnetically driven captive impeller or overhead agitation (magnetically coupled)
Stirrer speed	250 – 1500 rpm	Magnetically driven 250 – 1500 rpm · Overhead agitation 20 – 2000 rpm
Liquid additions with independent control for each bioreactor	2 as standard, options for up to 2 additional liquid feeds
Gas additions	1 set of 8 (100) or 16 (100XL) mass flow controllers, option for 1 additional set	1 set of 4 (400) or 8 (400XL) mass flow controllers, option for 1 additional set
Standard options for MFC calibration	Air, oxygen as option
Gas spargers	1
Exhaust gas	Peltier cooled condenser
pH control	1-way control (2-way control optional)
pH sensing	0 – 14
Polarographic DO sensing	Standard
DO control	Gas/liquid flow rate, stirrer speed
BioVIS cell-density monitoring	Optional
Tandem off-gas analysis	Optional
Foam/Level detection	Optional
Control PC and software	WinISO control software (pre-installed)

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Highlighted rows are where the two configurations differ — everything else is common to both.

Download the biotechnology brochure →

Ask a specialist about the BioXplorer 100 / 400 Compare the range side by side ↓↑ Back to top

High pressure

The BioXplorer P series — parallel screening at up to 10 bar

The parallel platform in stainless steel: discover and optimise gas-dependent bioprocesses at up to 10 bar — from syngas fermentation to cell-free enzymatics — with the same two configurations as the bench-top line. Fully customisable to your requirements.

8 reactors · 50–150 ml · 10 bar

BioXplorer 100P

The 100 in stainless steel: eight independent pressure bioreactors for high-throughput screening of gas-dependent bioprocesses — maximum experimental throughput at up to 10 bar.

4 reactors · 120–400 ml · 10 bar

BioXplorer 400P

Four independent stainless steel pressure bioreactors where working volume matters — with dual gas spargers per reactor and the choice of magnetic stirring or overhead agitation for demanding cultures.

Shared across both configurations

Stainless steel bioreactors, run in parallel or independently, at up to 10 bar
0 – 135 °C with 0.1 °C temperature resolution
2 sets of mass flow controllers as standard, with calibration options for air, oxygen, nitrogen, hydrogen, carbon monoxide, carbon dioxide or methane
Two gas spargers per reactor; autoclavable including sensors
Modular probes, gas and liquid feeds specified around your application

Run gas-dependent bioprocesses under pressure — before committing to scale.

Syngas fermentationCell-free biocatalysisC1 gas fermentationCO₂ biomanufacturingHigh-density cultivation

Stainless steel high-pressure reactors for the P series — Stainless steel HP reactors — 100P & 400P

High-pressure reactor with probes and gas connections — HP reactor with probes and gas connections

High-pressure reactor vessel and lid detail — Pressure vessel and lid detail

Glass vessel and stainless steel high-pressure vessel — Glass (standard) and stainless (P) vessels

Specifications

Specification	BioXplorer 100P	BioXplorer 400P
Number of independent parallel bioreactors	8	4
Total volume	200 ml	500 ml
Working volume	50 – 150 ml	120 – 400 ml
Bioreactor material	Stainless steel body and lid
Pressure rating	Up to 10 bar
Sterilization	Autoclavable bioreactors (including sensors)
Temperature range	0 – 135 °C
Temperature resolution	0.1 °C
Stirrer drive	Magnetically driven captive impeller	Magnetically driven captive impeller or overhead agitation (magnetically coupled)
Stirrer speed	250 – 1500 rpm	Magnetically driven 250 – 1500 rpm · Overhead agitation 20 – 2000 rpm
Gas additions	2 sets of 8 mass flow controllers as standard, option for 2 additional sets	2 sets of 4 mass flow controllers as standard, option for 2 additional sets
Standard options for MFC calibration	Air, oxygen, nitrogen, hydrogen, carbon monoxide, carbon dioxide or methane
Gas spargers	2
pH control	1-way control (2-way control optional)
pH sensing	0 – 14
Polarographic DO sensing	Optional
BioVIS cell-density monitoring	Optional
Tandem off-gas analysis	Optional
Foam/Level detection	Optional
Control PC and software	WinISO control software (pre-installed)

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Highlighted rows are where the two configurations differ — everything else is common to both.

Download the biotechnology brochure →

Ask a specialist about the BioXplorer 100P / 400P Compare the range side by side ↓↑ Back to top

Scale-up & custom

The BioXplorer 5000 — single-reactor scale-up to 5 litres

Drive your scale-up efficiency with a single, highly automated bioreactor in two variants — closely mimic production-scale conditions in early development, from standard aerobic cultures to high-pressure C1 gas fermentation. Fully customisable to your requirements.

1–5 L · glass · ambient pressure

BioXplorer 5000

Interchangeable insulated glass vessels from 1 to 5 litres at ambient pressure — the standard platform for aerobic cultures, high-density cultivation and process scale-up.

1–5 L · stainless steel · up to 10 bar

BioXplorer 5000P

The same platform with stainless steel cylinders rated to 10 bar — explore pressure effects on your bioprocess before committing to scale, from syngas fermentation to high-pressure C1 gas fermentation.

Shared across both variants

Single, highly automated bioreactor with interchangeable insulated vessels, 1 – 5 L
−50 to 250 °C with fluctuations typically < ±0.1 °C
Automated liquid & gas feeds, active-pump or multisampling (ASU) sampling
Integrates FTIR, DO, particle-sizing and Raman alongside standard sensing
WinISO control — unlimited step-recipe planning, data logging and ELN export

Closely mimic production-scale conditions in early development.

Process scale-upSyngas fermentationHigh-pressure C1 gas fermentationAerobic culturesCell-free biocatalysis

Specifications

Specification	BioXplorer 5000	BioXplorer 5000P
Number of reactors	Single reactor
Vessel material	Glass — interchangeable insulated vessels	Stainless steel cylinders
Pressure	Ambient	Up to 10 bar
Working volume	1 – 5 L
Temperature range	−50 to 250 °C (circulator dependent) · fluctuations typically < ±0.1 °C
Stirrer speed	Up to 2000 rpm / 400 Ncm
Sampling	Active pumps, manual dip tubes, automated level control or multisampling (ASU)
Reagent addition	Automated liquid & gas feeds; volumetric syringe pumps
Sensors	Temperature, pressure, pH, turbidity, conductivity; integrates FTIR, DO, particle-sizing and Raman
Off-gas analysis	Tandem off-gas analysis — OUR, CPR and RQ
Cell density	BioVIS — online monitoring without sampling
Control PC & software	Windows PC with WinISO / H.E.L control software — unlimited step-recipe planning, data logging and ELN export

MoreLess

Highlighted rows are where the two variants differ — everything else is common to both.

Download the biotechnology brochure →

Ask a specialist about the BioXplorer 5000 / 5000P Compare the range side by side ↓↑ Back to top

Compare the BioXplorer range

BioXplorer 100 / 100XL

Maximum screening throughput at the smallest practical volume

Parallel bioreactors: 8 (100) · 16 (100XL)
Working volume: 50–150 ml
Vessel: glass, autoclavable including sensors

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BioXplorer 400 / 400XL

Parallel screening where working volume matters

Parallel bioreactors: 4 (400) · 8 (400XL)
Working volume: 120–400 ml
Vessel: glass, autoclavable including sensors

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BioXplorer 100P / 400P

Gas-dependent bioprocesses under pressure

Parallel bioreactors: 8 (100P) · 4 (400P)
Working volume: 50–150 ml · 120–400 ml
Vessel: stainless steel, up to 10 bar

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BioXplorer 5000 / 5000P

Single-reactor scale-up at production-like conditions

Reactors: single, 1–5 L interchangeable vessels
Vessel: glass (5000) · stainless steel, up to 10 bar (5000P)
Temperature: −50 to 250 °C

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Comparing your selection

BioXplorer 100 / 100XL

Parallel bioreactors8 (100) · 16 (100XL)

Working volume50–150 ml

VesselGlass, autoclavable including sensors

PressureAmbient

AgitationMagnetic, 250–1500 rpm

Temperature0–135 °C · 0.1 °C resolution

Gas handling1 set of 8/16 MFCs · air, O₂ option

MonitoringpH + DO standard · BioVIS, Tandem, foam/level optional

SoftwareWinISO, pre-installed

BioXplorer 400 / 400XL

Parallel bioreactors4 (400) · 8 (400XL)

Working volume120–400 ml

VesselGlass, autoclavable including sensors

PressureAmbient

AgitationMagnetic 250–1500 rpm or overhead 20–2000 rpm

Temperature0–135 °C · 0.1 °C resolution

Gas handling1 set of 4/8 MFCs · air, O₂ option

MonitoringpH + DO standard · BioVIS, Tandem, foam/level optional

SoftwareWinISO, pre-installed

BioXplorer 100P / 400P

Parallel bioreactors8 (100P) · 4 (400P)

Working volume50–150 ml · 120–400 ml

VesselStainless steel body and lid

PressureUp to 10 bar

AgitationMagnetic 250–1500 rpm or overhead 20–2000 rpm

Temperature0–135 °C · 0.1 °C resolution

Gas handling2 MFC sets · air to H₂, CO, CO₂, methane · dual spargers

MonitoringpH standard · DO, BioVIS, Tandem, foam/level optional

SoftwareWinISO, pre-installed

BioXplorer 5000 / 5000P

ReactorsSingle · 1–5 L interchangeable vessels

Working volume1–5 L

VesselGlass (5000) · stainless steel (5000P)

PressureAmbient (5000) · up to 10 bar (5000P)

AgitationUp to 2000 rpm / 400 Ncm

Temperature−50 to 250 °C

Gas handlingAutomated gas & liquid feeds, syringe pumps

MonitoringpH, DO, redox · BioVIS, Tandem · FTIR, particle-sizing, Raman

SoftwareWinISO — recipes, logging, ELN export

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From first screen to first scale-up — one platform.

Every BioXplorer runs on the same WinISO control, so methods and data move with you from 50 ml to 5 litres.

Configure it your way

Every BioXplorer is specified around your process — probes, feeds, gas handling and analytics are chosen for your application, not the other way round.

BioVIS cell density

Inline, non-invasive monitoring of total cell growth and biomass — no sampling required.

View BioVIS →Brochure (PDF) →

Tandem off-gas analysis

Real-time CO₂ and O₂ analysis for OUR, CPR and RQ — multi-line and continuous systems.

View Tandem →Brochure (PDF) →

Gas & liquid feeds

Independent mass flow controllers per reactor and automated liquid additions — calibrations from air and O₂ to H₂, CO, CO₂ and methane.

Sensors & sampling

pH, DO, redox, foam/level and multisampling (ASU); integrates FTIR, particle-sizing and Raman on scale-up.

Selected publications

Optimising docosahexaenoic fatty acid production by Crypthecodinium cohniiChalima et al. · Bioresource Technology Reports · 2022Statistical optimisation of biomass and exopolysaccharide production by Lacticaseibacillus rhamnosus LRH30Copeland et al. · World J. Microbiology & Biotechnology · 2025High pressure / high temperature, multi-sensing bioreactor system for microbial risk assessment in underground hydrogen storageVasile, Bellini, Bassani et al. · Int. J. Hydrogen Energy · 2024Versioning biological cells for trustworthy cell engineeringTellechea-Luzardo et al. · Nature Communications · 2022Isobutanol production freed from biological limits using synthetic biochemistrySherkhanov, Korman, Chan et al. · Nature Communications · 2020Automated bioprocess feedback via a mobile robotic lab assistantKaspersetz et al. · Frontiers in Chemical Engineering · 2022

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Complete the platform

BioVIS

Inline, non-invasive cell-density monitoring

View →

Tandem Gas Analysers

Real-time off-gas analysis of CO₂ and O₂

View →

Frequently asked questions

Which BioXplorer is right for my process?

Start from throughput and scale: the BioXplorer 100 gives eight reactors at 50–150 ml — or sixteen on the 100XL — for maximum screening throughput; the 400 gives four at 120–400 ml, or eight on the 400XL. For gas-dependent work under pressure, the 100P and 400P run the same two configurations in stainless steel at up to 10 bar; for scale-up, the 5000 is a single 1–5 L bioreactor — glass at ambient pressure, or stainless steel at up to 10 bar (5000P). A specialist can help you match the platform to your application.

Is the system customisable to my process?

Yes. Every BioXplorer is configured to your requirements — vessels, probes, sensors, gas and liquid feeds are all specified around your application. Customisation to the customer’s process is central to H.E.L’s approach.

What working volumes are available?

From 50 ml to 5 L across the family: 50–150 ml (100 and 100XL), 120–400 ml (400, 400XL and the P variants) and 1–5 L interchangeable vessels (5000) — so methods developed at screening scale carry through to scale-up.

Can I run gas-dependent bioprocesses under pressure?

Yes. The BioXplorer 100P and 400P run stainless-steel reactors (eight or four) at up to 10 bar with dual gas spargers per reactor and MFC calibrations for air, O₂, N₂, H₂, CO, CO₂ and methane; the 5000P scales the same capability to 5 L. Typical applications include syngas fermentation, CO₂ biomanufacturing and Power-to-Liquid.

What does the control software do?

The H.E.L WinISO software runs every reactor with fully independent, automated control — advanced strategies from sensor feedback, time-based profiles or event detection, with unlimited step-recipe planning, real-time logging, .csv export and ELN integration.

What monitoring and analytics options are available?

pH and dissolved-oxygen sensing as standard; optional BioVIS for online cell-density monitoring without sampling, foam/level detection, and Tandem off-gas analysis for OUR, CPR and RQ. The 5000 also integrates FTIR, particle-sizing and Raman.

Are the bioreactors autoclavable?

Yes — the parallel platforms use autoclavable bioreactors, including sensors, with glass bodies and stainless-steel lids (fully stainless on the P variants).

Ready to accelerate your research?

Talk to a bioprocess specialist about your application.

Ask a specialist Download specifications book Biotechnology brochure

The BioXplorer 100 & 400 series — parallel screening on the bench

BioXplorer 100 / 100XL

BioXplorer 400 / 400XL

Shared across both configurations

Specifications

The BioXplorer P series — parallel screening at up to 10 bar

BioXplorer 100P

BioXplorer 400P

Shared across both configurations

Specifications

The BioXplorer 5000 — single-reactor scale-up to 5 litres

BioXplorer 5000

BioXplorer 5000P

Shared across both variants

Specifications

Compare the BioXplorer range

BioXplorer 100 / 100XL

BioXplorer 400 / 400XL

BioXplorer 100P / 400P

BioXplorer 5000 / 5000P

Comparing your selection

BioXplorer 100 / 100XL

BioXplorer 400 / 400XL

BioXplorer 100P / 400P

BioXplorer 5000 / 5000P

From first screen to first scale-up — one platform.

Configure it your way

BioVIS cell density

Tandem off-gas analysis

Gas & liquid feeds

Sensors & sampling

Selected publications

Related reading

Complete the platform

BioVIS

Tandem Gas Analysers

Frequently asked questions

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