BioXplorer 400
Parallel bioreactor screening, up to 400 mL
Optimise bioprocesses across four independent bioreactors with working volumes up to 400 mL — highly automated and fully customisable to your requirements.
4
Parallel reactors
120–400 mL
Working volume
Automated
Control
Real-time
Monitoring
0–130 °C
Temperature
up to 2000 rpm
Agitation
Why choose the BioXplorer 400?
Faster development
Run experiments in parallel and accelerate optimisation.
Lower costs
Modest working volumes cut media and reagent use.
Better control
Independent pH, DO, temperature and feeding in every reactor.
Actionable insights
Real-time data and WinISO tools for faster, data-driven decisions.
Specifications
| Number of independent parallel bioreactors | 4 |
|---|---|
| Bioreactor material | Glass body, stainless steel lid |
| Total volume | 500ml |
| Working volume | 120 – 400 ml |
| Sterilization | Autoclavable bioreactors (including sensors) |
| Temperature Range | 0 – 130 °C |
| Temperature resolution | 0.1 °C |
| Stirrer drive | Magnetically driven captive impeller or Overhead agitation (magnetically coupled) |
| Stirrer speed | Magnetically Driven – 250 – 1500 rpm Overhead Agitation – 20- 2000rpm |
| Liquid additions with independent control for each bioreactor | 2 as standard, options for up to 2 additional liquid feeds |
| Gas additions | 1 set of 4 mass flow controllers allowing independent control for each reactor as standard, option for 1 additional set |
| Standard options for MFC calibration | Air, oxygen as option |
| Gas spargers | 1 |
| Exhaust gas | Peltier cooled condenser |
| pH control | 1-way control (2-way control optional) |
| pH Sensing | 0-14 |
| Polarographic DO sensing | Standard |
| DO control | Gas/liquid flow rate, stirrer speed |
| BioVIS cell-density monitoring | Optional |
| Tandem off-gas analysis | Optional |
| Foam/Level detection | Optional |
| Control PC and software | WinISO control software (pre-installed) |
Software
The H.E.L WinISO software runs every reactor with fully independent, automated control — advanced strategies from sensor feedback, time-based profiles or event detection, with real-time logging and .csv export.
Fully customisable, it controls the complete range of process parameters — temperature, stirrer speed, dissolved oxygen, pH, gas flow rates, liquid feed rates and foam & level detection.
Applications
Frequently asked questions
What is the BioXplorer 400 used for?
A bench-top, four-reactor platform for parallel bioprocess optimisation and screening — aerobic fermentation, high-density cultivation, yeast and gas fermentation, and SAF development.
Is the system customisable to my process?
Yes. Every BioXplorer 400 is configured to your requirements — probes, sensors, gas and liquid feeds are all specified around your application. Customisation to the customer’s process is central to H.E.L’s approach.
How many reactors and what working volume?
Four parallel, independently-controlled bioreactors with working volumes of 120–400 ml (glass body, stainless-steel lid, fully autoclavable including sensors).
What temperature and agitation does it offer?
0 to 130 °C with 0.1 °C resolution, with a choice of magnetic stirring (250–1500 rpm) or overhead agitation (20–2000 rpm).
What does the control software do?
The H.E.L WinISO software gives fully independent control of all four reactors — automated stirring, gas and liquid additions from sensor feedback, with real-time logging and .csv export.
What monitoring options are available?
pH (0–14) and polarographic dissolved-oxygen sensing as standard; optional BioVIS for online cell-density monitoring and foam/level detection; optional Tandem off-gas analysis for OUR, CPR and RQ.
Selected publications
Statistical optimisation of biomass and exopolysaccharide production by Lacticaseibacillus rhamnosus LRH30Copeland et al. · World J. Microbiology & Biotechnology · 2025Cadmium effects on net N₂O production by Shewanella loihica PV-4Pizarro et al. · FEMS Microbiology Letters · 2023Versioning biological cells for trustworthy cell engineeringTellechea-Luzardo et al. · Nature Communications · 2022
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Related reading
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Talk to a bioprocess specialist about your application.
