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BioXplorer 400 | bench-top, parallel 4 bioreactor platform

The BioXplorer 400 boosts productivity and enables screening of cell culture conditions in larger culture volumes. The modular design and extensive list of options make the BioXplorer 400 adaptable to a wide range of applications, including microbial fermentation, C1 Gas fermentation and cell culture. This makes the BioXplorer 400 suitable for process development, Design of Experiment (DoE) studies, or essential research.

With fully automated control of either four or eight bioreactors, the BioXplorer 400 supports cell culture working volumes of between 20 ml and 400 ml. The individual control of each reactor enables the running of replicate samples with unique conditions, or any combination that your experiment requires. Multiple systems can be run from the same control PC for extended experiments.

Offering

– Parallel processing of 4 independent reactors, in a small footprint
– Choice of reactor sizes and types including elevated pressure design (5 bar or 10 bar options)
– Fully automated parallel processing of up to 4 independent reactors
– Integrated individual liquid and gas feeds with options for gas mixing

Features

Bioreactors

We supply a range of different bioreactors compatible with the BioXplorer 400 system.

Reusable Glass bioreactors are suitable for a wide range of applications at atmospheric pressure
Stainless steel reactors for standard or elevated pressure (up to 10 bar / 145 PSI) fermentations
Specially designed head plates to accommodate all probes and feeds
Working volumes from 20 ml to 400 ml
- Smaller vessels are suitable for data-rich screening campaigns
- Larger vessels support scale-up requirements
Bioreactor geometry that mimics a large scale system

The H.E.L control software runs the BioXplorer 400 system, enabling complex, fully automated experiments to run unattended. This enables:

Multi reactor control with unlimited step recipe planning. Plans can be changed online at any time without process interruption
Starting and stopping reactors individually or simultaneously
The individual reactor temperature and agitation control
User-defined data-logging with customizable tabular and graphical displays
Export data functions and integration with in-house electronic lab notebooks and record-keeping systems
DoE and QbD package integration

More specifically, the software controls a wide range of system features, including:

Temperature
Gas feeds, flow and mix
pH
Dissolved oxygen
Redox
Stirrer speed
Torque (for viscosity indication)
Pressure
Liquid feed rates
Antifoam and level detection

Options

Gas Feeds

Choose from simple or more sophisticated gas flow control regimes
Each bioreactor can be configured with single or multiple mass flow controllers
For microbial fermentations, automatic oxygen enrichment is possible
For cell culture applications CO2 can be used for pH control
Ratios of several gases can be mixed and regulated with online feedback from off-gas analytics

Liquid Feeds

Choice of liquid feeds for standard and pressure applications
Highly linear peristaltic pump drives are integrated into standard heads which enable exceptional pH and dosing control as well as a wide operating range
Typically, two to four pumps per reactor are supplied but additional pumps can be added as needed
Syringe pumps and high-pressure pumps are also available

Magnetically Suspended Stirring

Ideally suited to small bioreactors with proven performance
No shaft seal or moving parts: no sterility issues
Excellent magnetic coupling
Range of stirrer designs (Marine, Rushton, etc)
Speed options of 300 rpm to 1500 rpm or 30 rpm to 200 rpm

pH, Dissolved Oxygen and Redox

A wide range of probes are available to cater for different culture types, reactor sizes and applications
All probes are integrated into the WinISO software to allow automated monitoring and feedback loops

Off Gas Analysis

Tandem off-gas analysis units monitor and control a culture process continuously
This enables automatic calculation of oxygen uptake rate (OUR); carbon dioxide production rate (CPR) and respiration quotient (RQ)

Cell Density and Biomass Monitoring

BioVIS enables the online monitoring of cell growth and biomass using optical density, removing the need for online sampling
Track cell growth and understand the processes without taking samples
Viable cell density probes can also be integrated

Publications

The following are a list of some technical publications which highlight the use of the equipment.

Versioning biological cells for trustworthy cell engineering

Jonathan Tellechea-Luzardo, Leanne Hobbs, Elena Velázquez, Lenka Pelechova, Simon Woods, Víctor de Lorenzo and Natalio Krasnogor

09-Feb-2022

https://www.nature.com/articles/s41467-022-28350-4(Subscription or purchase maybe required for full access)

Harnessing Escherichia coli for bio-based production of formate under pressurized H2 and CO2 gases

M.Roger, T.C. Reed, F. Sargent

01-Jan-2021

https://doi.org/10.1101/2021.01.06.425572(Subscription or purchase maybe required for full access)

Validation and Optimization of a Yeast Dynamic Flux Balance Model using a Parallel Bioreactor System

T. J. Hanly, A.R. Tiernan, M.A. Henson

01-Dec-2013

https://doi.org/10.3182/20131216-3-IN-2044.00002(Subscription or purchase maybe required for full access)

BioXplorer 400 | bench-top, parallel 4 bioreactor platform