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BioXplorer 400 | bench-top, parallel 4 bioreactor platform

The BioXplorer 400 boosts productivity and enables screening of cell culture conditions in larger culture volumes.  The modular design and extensive list of options make the BioXplorer 400 adaptable to a wide range of applications, including microbial fermentation, C1 Gas fermentation and cell culture.  This makes the BioXplorer 400 suitable for process development, Design of Experiment (DoE) studies, or essential research.

With fully automated control of either four or eight bioreactors, the BioXplorer 400 supports cell culture working volumes of between 20 ml and 400 ml.  The individual control of each reactor enables the running of replicate samples with unique conditions, or any combination that your experiment requires.  Multiple systems can be run from the same control PC for extended experiments.


– Parallel processing of 4 independent reactors, in a small footprint
– Choice of reactor sizes and types including elevated pressure design (5 bar or 10 bar options)
– Fully automated parallel processing of up to 4 independent reactors
– Integrated individual liquid and gas feeds with options for gas mixing



We supply a range of different bioreactors compatible with the BioXplorer 400 system.

  • Reusable Glass bioreactors are suitable for a wide range of applications at atmospheric pressure
  • Stainless steel reactors for standard or elevated pressure (up to 10 bar / 145 PSI) fermentations
  • Specially designed head plates to accommodate all probes and feeds
  • Working volumes from 20 ml to 400 ml
    • Smaller vessels are suitable for data-rich screening campaigns
    • Larger vessels support scale-up requirements
  • Bioreactor geometry that mimics a large scale system



The H.E.L control software runs the BioXplorer 400 system, enabling complex, fully automated experiments to run unattended. This enables:

  • Multi reactor control with unlimited step recipe planning. Plans can be changed online at any time without process interruption
  • Starting and stopping reactors individually or simultaneously
  • The individual reactor temperature and agitation control
  • User-defined data-logging with customizable tabular and graphical displays
  • Export data functions and integration with in-house electronic lab notebooks and record-keeping systems
  • DoE and QbD package integration


More specifically, the software controls a wide range of system features, including:

  • Temperature
  • Gas feeds, flow and mix
  • pH
  • Dissolved oxygen
  • Redox
  • Stirrer speed
  • Torque (for viscosity indication)
  • Pressure
  • Liquid feed rates
  • Antifoam and level detection


Gas Feeds

  • Choose from simple or more sophisticated gas flow control regimes
  • Each bioreactor can be configured with single or multiple mass flow controllers
  • For microbial fermentations, automatic oxygen enrichment is possible
  • For cell culture applications CO2 can be used for pH control
  • Ratios of several gases can be mixed and regulated with online feedback from off-gas analytics


Liquid Feeds

  • Choice of liquid feeds for standard and pressure applications
  • Highly linear peristaltic pump drives are integrated into standard heads which enable exceptional pH and dosing control as well as a wide operating range
  • Typically, two to four pumps per reactor are supplied but additional pumps can be added as needed
  • Syringe pumps and high-pressure pumps are also available


Magnetically Suspended Stirring

  • Ideally suited to small bioreactors with proven performance
  • No shaft seal or moving parts: no sterility issues
  • Excellent magnetic coupling
  • Range of stirrer designs (Marine, Rushton, etc)
  • Speed options of 300 rpm to 1500 rpm or 30 rpm to 200 rpm


pH, Dissolved Oxygen and Redox

  • A wide range of  probes are available to cater for different culture types, reactor sizes and applications
  • All probes are integrated into the WinISO software to allow automated monitoring and feedback loops


Off Gas Analysis

  • Tandem off-gas analysis units monitor and control a culture process continuously
  • This enables automatic calculation of oxygen uptake rate (OUR);  carbon dioxide production rate (CPR) and respiration quotient (RQ)


Cell Density and Biomass Monitoring

  • BioVIS enables the online monitoring of cell growth and biomass using optical density, removing  the need for online sampling
  • Track cell growth and understand the processes without taking samples
  • Viable cell density probes can also be integrated



The following are a list of some technical publications which highlight the use of the equipment.

Versioning biological cells for trustworthy cell engineering

Jonathan Tellechea-Luzardo, Leanne Hobbs, Elena Velázquez, Lenka Pelechova, Simon Woods, Víctor de Lorenzo and Natalio Krasnogor


https://www.nature.com/articles/s41467-022-28350-4(Subscription or purchase maybe required for full access)

Harnessing Escherichia coli for bio-based production of formate under pressurized H2 and CO2 gases

M.Roger, T.C. Reed, F. Sargent


https://doi.org/10.1101/2021.01.06.425572(Subscription or purchase maybe required for full access)

Validation and Optimization of a Yeast Dynamic Flux Balance Model using a Parallel Bioreactor System

T. J. Hanly, A.R. Tiernan, M.A. Henson


https://doi.org/10.3182/20131216-3-IN-2044.00002(Subscription or purchase maybe required for full access)

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