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BioXplorer 100 | bench-top, parallel 8 bioreactor platform

The BioXplorer 100 is a bench-top, parallel 8 bioreactor platform, typically with 125 ml volume reactors. It is typically utilized in:

Research
The latter stages of screening / early stages of process development

It has the same design, inputs, and outputs as larger systems, thus facilitating a greater number of investigative variables than a microplate or shake flask, but with an increased number of parallel samples than larger bioreactor systems. This combination of relatively high throughput with high information content enables better decision-making for scale-up and thus can speed up development.

The BioXplorer 100 is available as both a low- and high-pressure variant, enabling access to a wide range of applications ranging from standard gas fermentation and aerobic cultures to facilitating studies into decarbonizing high-pressure C1 gas fermentation.

Features and Options

Vessel Types and Volumes

Reusable glass bioreactors are appropriate for a wide range of applications at atmospheric pressure.
All reactors are suitable for autoclaving, with auto-cleavable probes as standard.
Uniquely developed head plates to house all feeds and probes.
Working volumes ranging from 20 to 100 mL.
Bioreactor geometry that mimics production-scale systems.

Temperature Control

-50 ⁰C to 250 ⁰C as standard (circulator dependent).
Fine temperature control, with fluctuations normally lower than ±0.05 ⁰C

High-Pressure Systems

Stainless steel reactors for standard or elevated pressure (up to 10 bar/145 PSI) fermentations.

Sampling Systems

Through active pumps, manual dip tubes, automated level control, or multisampling units, like the ASU.

Sensor Options

Temperature, pressure, pH, DO, turbidity, conductivity.
Optional: Integration of third-party probes, such as in-situ FTIR, particle sizing probes, and Raman probes.

Independent Stirring

250 up to 1500 rpm via suspended magnetic agitation.
0 up to 1500 rpm via overhead stirring.

Reagent Addition

Through fully automated liquid and gas feeds as well as volumetric syringe pumps. These solutions can be applied to both high and low-pressure systems.

Control Options

All relevant inputs can be logged, enabling automated monitoring and, where appropriate, feedback control of the reactor system

Intelligent Software Control and Analysis

The control software from H.E.L operates the BioXplorer 100 system, thereby allowing complicated, completely automated experiments to run without the need to be monitored. This allows:
- Multi reactor control with unrestricted step recipe planning, where plans can be altered at any time without process interruption
- User defined data logging with customizable tabular and graphical displays
- Starting and stopping reactors simultaneously or individually
- Export data functions and integration with in-house record keeping systems and electronic lab notebooks

Safety Features

Include automatic user-configurable reaction monitoring and shutdown procedures, to ensure user safety.

Automatic hardware and software fail-safes are installed on every system.

Resources

Technical Literature:

Blogs:

Publications

The following are a list of some technical publications which highlight the use of the equipment.

Optimizing the production of docosahexaenoic fatty acid by Crypthecodinium cohnii and reduction in process cost by using a dark fermentation effluent

Angelina Chalima, Christos Boukouvalas, Vasiliki Oikonomopoulou and Evangelos Topakas

15-Aug-2022

https://www.sciencedirect.com/science/article/pii/S2666821122001053(Subscription or purchase maybe required for full access)

Studying semi-dynamic digestion kinetics of food: Establishing a computer-controlled multireactor approach

S.H.E. Verkempinck, D. Duijsens, D. Michels, J.M. Guevara-Zambrano, M.R. Infantes-Garcia, K. Pälchen, T. Grauwet

01-Jun-2022

https://www.sciencedirect.com/science/article/abs/pii/S0963996922003581(Subscription or purchase maybe required for full access)

Automated Bioprocess Feedback Operation in a High-Throughput Facility via the Integration of a Mobile Robotic Lab Assistant

Lucas Kaspersetz, Saskia Waldburger, M.-Therese Schermeyer, Sebastian L. Riedel, Sebastian Groß, Peter Neubauer and M.-Nicolas Cruz-Bournazou

07-Apr-2022

https://www.frontiersin.org/articles/10.3389/fceng.2022.812140/full(Subscription or purchase maybe required for full access)

In vitro fermentation test bed for evaluation of engineered probiotics in polymicrobial communities

S. Arcidiancono, A.M. Ehrenworth-Breedon, M.S. Goodson, L.A. Doherty, W. Lyon, G. Jimenez, I.G. Pantoja-Feliciano, J.W. Soares

01-May-2021

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8175340/(Subscription or purchase maybe required for full access)

Dietary vitamin K is remodeled by gut microbiota and influences community composition

J.L. Ellis, J. Philip Karl, A.M. Oliverio, X. Fu, J.W. Soares, B.E. Wolfe, C.J. Hernandez, J. B. Mason, S.L. Booth

01-Mar-2021

https://doi.org/10.1080/19490976.2021.1887721(Subscription or purchase maybe required for full access)

Farm in a Lab: Cultivating A Lab-Grown Meat Solution (PhD thesis)

M.Forcier, J.Ganoe, E.Hall, H.Larochelle

01-May-2020

https://web.wpi.edu/Pubs/E-project/Available/E-project-051320-180900/unrestricted/Farm_in_a_Lab_MQP_Report.pdf(Subscription or purchase maybe required for full access)

Acute stressor alters inter-species microbial competition for resistantstarch-supplemented medium

Ida Gisela Pantoja-Feliciano, Jason W. Soares, Laurel A. Doherty, J. Philip Karl, Holly L. McClung, Nicholes J. Armstrong, Tobyn A. Branck and Steven Arcidiacono

22-Dec-2018

https://www.tandfonline.com/doi/full/10.1080/19490976.2018.1554962(Subscription or purchase maybe required for full access)