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BioXplorer 100 | bench-top, parallel 8 bioreactor platform

The BioXplorer 100 is a bench-top, parallel 8 bioreactor platform, typically with 125 ml volume reactors.  It is typically utilized in:

  • Research
  • The latter stages of screening / early stages of process development

It has the same design, inputs, and outputs as larger systems, thus facilitating a greater number of investigative variables than a microplate or shake flask, but with an increased number of parallel samples than larger bioreactor systems. This combination of relatively high throughput with high information content enables better decision-making for scale-up and thus can speed up development.

The BioXplorer 100 is available as both a low- and high-pressure variant, enabling access to a wide range of applications ranging from standard gas fermentation and aerobic cultures to facilitating studies into decarbonizing high-pressure C1 gas fermentation.

Features and Options

Vessel Types and Volumes

  • Reusable glass bioreactors are appropriate for a wide range of applications at atmospheric pressure.
  • All reactors are suitable for autoclaving, with auto-cleavable probes as standard.
  • Uniquely developed head plates to house all feeds and probes.
  • Working volumes ranging from 20 to 100 mL.
  • Bioreactor geometry that mimics production-scale systems.

Temperature Control

  • -50 ⁰C to 250 ⁰C as standard (circulator dependent).
  • Fine temperature control, with fluctuations normally lower than ±0.05 ⁰C

High-Pressure Systems

  • Stainless steel reactors for standard or elevated pressure (up to 10 bar/145 PSI) fermentations.

Sampling Systems

  • Through active pumps, manual dip tubes, automated level control, or multisampling units, like the ASU.

Sensor Options

  • Temperature, pressure, pH, DO, turbidity, conductivity.
  • Optional: Integration of third-party probes, such as in-situ FTIR, particle sizing probes, and Raman probes.

Independent Stirring

  • 250 up to 1500 rpm via suspended magnetic agitation.
  • 0 up to 1500 rpm via overhead stirring.

Reagent Addition

  • Through fully automated liquid and gas feeds as well as volumetric syringe pumps. These solutions can be applied to both high and low-pressure systems.

Control Options

  • All relevant inputs can be logged, enabling automated monitoring and, where appropriate, feedback control of the reactor system

Intelligent Software Control and Analysis

  • The control software from H.E.L operates the BioXplorer 100 system, thereby allowing complicated, completely automated experiments to run without the need to be monitored. This allows:
    • Multi reactor control with unrestricted step recipe planning, where plans can be altered at any time without process interruption
    • User defined data logging with customizable tabular and graphical displays
    • Starting and stopping reactors simultaneously or individually
    • Export data functions and integration with in-house record keeping systems and electronic lab notebooks

Safety Features

  • Include automatic user-configurable reaction monitoring and shutdown procedures, to ensure user safety.

Automatic hardware and software fail-safes are installed on every system.


Technical Literature:



The following are a list of some technical publications which highlight the use of the equipment.

Optimizing the production of docosahexaenoic fatty acid by Crypthecodinium cohnii and reduction in process cost by using a dark fermentation effluent

Angelina Chalima, Christos Boukouvalas, Vasiliki Oikonomopoulou and Evangelos Topakas


https://www.sciencedirect.com/science/article/pii/S2666821122001053(Subscription or purchase maybe required for full access)

Studying semi-dynamic digestion kinetics of food: Establishing a computer-controlled multireactor approach

S.H.E. Verkempinck, D. Duijsens, D. Michels, J.M. Guevara-Zambrano, M.R. Infantes-Garcia, K. Pälchen, T. Grauwet


https://www.sciencedirect.com/science/article/abs/pii/S0963996922003581(Subscription or purchase maybe required for full access)

Automated Bioprocess Feedback Operation in a High-Throughput Facility via the Integration of a Mobile Robotic Lab Assistant

Lucas Kaspersetz, Saskia Waldburger, M.-Therese Schermeyer, Sebastian L. Riedel, Sebastian Groß, Peter Neubauer and M.-Nicolas Cruz-Bournazou


https://www.frontiersin.org/articles/10.3389/fceng.2022.812140/full(Subscription or purchase maybe required for full access)

In vitro fermentation test bed for evaluation of engineered probiotics in polymicrobial communities

S. Arcidiancono, A.M. Ehrenworth-Breedon, M.S. Goodson, L.A. Doherty, W. Lyon, G. Jimenez, I.G. Pantoja-Feliciano, J.W. Soares


https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8175340/(Subscription or purchase maybe required for full access)

Dietary vitamin K is remodeled by gut microbiota and influences community composition

J.L. Ellis, J. Philip Karl, A.M. Oliverio, X. Fu, J.W. Soares, B.E. Wolfe, C.J. Hernandez, J. B. Mason, S.L. Booth


https://doi.org/10.1080/19490976.2021.1887721(Subscription or purchase maybe required for full access)

Farm in a Lab: Cultivating A Lab-Grown Meat Solution (PhD thesis)

M.Forcier, J.Ganoe, E.Hall, H.Larochelle


https://web.wpi.edu/Pubs/E-project/Available/E-project-051320-180900/unrestricted/Farm_in_a_Lab_MQP_Report.pdf(Subscription or purchase maybe required for full access)

Acute stressor alters inter-species microbial competition for resistantstarch-supplemented medium

Ida Gisela Pantoja-Feliciano, Jason W. Soares, Laurel A. Doherty, J. Philip Karl, Holly L. McClung, Nicholes J. Armstrong, Tobyn A. Branck and Steven Arcidiacono


https://www.tandfonline.com/doi/full/10.1080/19490976.2018.1554962(Subscription or purchase maybe required for full access)

Acute stressor alters inter-species microbial competition for resistant starch-supplemented medium

I.G. Pantoja-Feliciano, J.W. Soares, L.A. Doherty, J. Philip Karl, H.L. McClung, N.J. Armstrong, T.A. Branck, S. Arcidiancono


https://doi.org/10.1080/19490976.2018.1554962(Subscription or purchase maybe required for full access)

Engineering Robust Production Microbes for Large-Scale Cultivation

M.Wehrs, D.Tanjore, T.Eng, J. Lievense, T.R. Pray, A. Mukhopadhyay


https://doi.org/10.1016/j.tim.2019.01.006(Subscription or purchase maybe required for full access)

Acetate-detoxification of wood hydrolysates with alkali tolerant Bacillus sp. as a strategy to enhance the lipid production from Rhodosporidium toruloides

L. Matsakas, K. Novak, J. Enman, P. Christakopoulos, U. Rova


https://doi.org/10.1016/j.biortech.2017.04.002(Subscription or purchase maybe required for full access)

Mixing Time as a Criterion for Scale Translation of Cell-Culture Processes (Magazine)

M. H. Sani, F. Baganz


https://www.researchgate.net/profile/Mohd-Sani/publication/298787880_Mixing_time_as_a_criterion_for_scale_translation_of_cell-culture_processes/links/56eb7a5508ae2a58dc4a32f0/Mixing-time-as-a-criterion-for-scale-translation-of-cell-culture-processes.pdf#page=41(Subscription or purchase maybe required for full access)

Continuous screening system for inhibited enzyme catalysis: A membrane reactor approach

E. Lyagin, A. Drews, S. Bhattacharya, M.B. Ansorge-Schumacher, M. Kraume


https://doi.org/10.1002/biot.201000130(Subscription or purchase maybe required for full access)

Thermal profiling for parallel on-line monitoring of biomass growth in miniature stirred bioreactors

N. K. Gill, M. Appleton , G. J. Lye


https://doi.org/10.1007/s10529-008-9719-0(Subscription or purchase maybe required for full access)

Miniature bioreactors: current practices and future opportunities

F. Baganz and J.L.Betts


https://doi.org/10.1186/1475-2859-5-21(Subscription or purchase maybe required for full access)

Design and Characterisation of Parallel Miniature Bioreactors for Bioprocess Optimisation and Scale-up

N.K. Gill


https://discovery.ucl.ac.uk/id/eprint/1445974/1/U593299%20redacted.PDF(Subscription or purchase maybe required for full access)

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