BioXplorer 5000

Name: BioXplorer 5000 | Lab-scale bioreactor platform
Brand: H.E.L Group

Single-bioreactor scale-up, 1–5 L, up to 10 bar

Drive your scale-up efficiency with a single, highly automated bioreactor — 1–5 L, ambient to 10 bar — fully customisable to your requirements, from standard aerobic cultures to high-pressure C1 gas fermentation.

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Single

Reactor

1–5 L

Working volume

Up to 10 bar

Pressure

−50–250 °C

Temperature

Automated

Control

up to 2000 rpm

Agitation

Why choose the BioXplorer 5000?

Ambient to 10 bar

Explore pressure effects on your bioprocess before committing to scale-up.

Configurable to your process

Vessels, feeds, probes and off-gas analysis specified around your application.

Scale-up ready

Closely mimic production-scale conditions in early development.

Actionable insights

Real-time data and WinISO tools for faster, data-driven decisions.

Specifications

Number of reactors	Single reactor
Vessel material	Glass or stainless steel (stainless for high-pressure 5000P)
Working volume	1–5 L — interchangeable insulated vessels
Pressure	Ambient; up to 10 bar (5000P)
Temperature range	−50 to 250 °C (circulator dependent)
Temperature control	Fluctuations typically < ±0.1 °C
Stirrer speed	Up to 2000 rpm / 400 Ncm
Sampling	Active pumps, manual dip tubes, automated level control or multisampling (ASU)
Reagent addition	Automated liquid & gas feeds; volumetric syringe pumps
Sensors	Temperature, pressure, pH, turbidity, conductivity; integrates FTIR, DO, particle-sizing and Raman
Off-gas analysis	Tandem off-gas analysis — OUR, CPR and RQ
Cell density	BioVIS — online monitoring without sampling
Control PC & software	Windows PC with WinISO / H.E.L control software — unlimited step-recipe planning, data logging and ELN export

Software

The H.E.L WinISO software runs every reactor with fully independent, automated control — advanced strategies from sensor feedback, time-based profiles or event detection, with real-time logging and .csv export.

Fully customisable, it controls the complete range of process parameters — temperature, gas feeds, flow & mix, pH, dissolved oxygen, redox, stirrer speed, torque (viscosity), pressure, liquid feed rates and antifoam & level detection.

Applications

Syngas fermentationHigh-pressure C1 gas fermentationAerobic culturesCell-free biocatalysisProcess scale-up

Frequently asked questions

What is the BioXplorer 5000 used for?

A lab-scale single-bioreactor platform for early-stage bioprocess development and scale-up — from standard aerobic cultures to high-pressure C1 gas fermentation, syngas fermentation and cell-free biocatalysis.

Can the system be customised to my process?

Yes. Every BioXplorer 5000 is fully configured to your requirements — vessel type and volume, gas and liquid feeds, probes, off-gas analysis and software control are all specified around your application. Customisation to the customer’s process is central to H.E.L’s approach.

What working volumes are available?

Interchangeable insulated vessels from 1 to 5 L, in glass or stainless steel. Both suit ambient-pressure work.

What is the maximum pressure, and how does the 5000P differ?

The high-pressure 5000P uses stainless-steel vessels rated up to 10 bar — ideal for replicating production-scale pressures and elevated-headspace processes. The standard BioXplorer 5000 runs at ambient pressure.

What temperature range and agitation does it offer?

−50 to 250 °C (circulator dependent), with fluctuations typically under ±0.1 °C, and agitation up to 2000 rpm / 400 Ncm.

What can the control software do?

The H.E.L WinISO software runs fully automated, unattended experiments with unlimited step-recipe planning, live plan edits, data-logging and ELN export, plus DoE and QbD integration.

What analytics and monitoring options are available?

pH, dissolved oxygen and redox probes integrated with WinISO; BioVIS for online cell-density monitoring; and Tandem off-gas analysis for OUR, CPR and RQ.