BioXplorer 400P

Name: BioXplorer 400P | High-pressure bench-top, parallel 4 bioreactor platform
Brand: H.E.L Group

Fully customizable high-pressure parallel bioreactor screening, up to 10 bar

Discover and optimize gas-dependent bioprocesses across four parallel reactors at up to 10 bar — from syngas fermentation to cell-free enzymatics — fully customizable to your requirements.

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Parallel reactors

Up to 10 bar

Pressure

120–400 mL

Working volume

Automated

Control

0–135 °C

Temperature

up to 2000 rpm

Agitation

Why choose the BioXplorer 400P?

High-pressure capability

Run gas-dependent bioprocesses across four reactors at up to 10 bar to increase gas solubility in the liquid.

Configurable to your process

Modular probes, gas and liquid feeds specified around your application.

Parallel efficiency

Operate four reactors in parallel or independently to cut optimization time.

Actionable insights

Real-time data and WinISO tools for faster, data-driven decisions.

Specifications

Number of independent parallel bioreactors	4
Bioreactor material	Stainless steel body and lid
Total volume	500ml
Working volume	120 – 400 ml
Pressure rating	Up to 10 bar
Sterilization	Autoclavable bioreactors (including sensors)
Temperature Range	0 – 135 °C
Temperature resolution	0.1 °C
Stirrer drive	Magnetically driven captive impeller or Overhead agitation (magnetically coupled)
Stirrer speed	Magnetically Driven – 250 – 1500 rpm Overhead Agitation – 20- 2000rpm
Auto re-couple	Magnetically Driven – Yes Overhead Agitation – N/A
Liquid additions with independent control for each bioreactor	2 as standard, options for up to 2 additional liquid feeds Overhead Agitation – 2 as standard, option for 1 additional liquid feed
Gas additions	2 sets of 4 mass flow controllers allowing independent control for each reactor as standard, option for 2 additional sets
Standard options for MFC calibration	Air, oxygen, nitrogen, hydrogen, carbon monoxide, carbon dioxide or methane
Gas spargers	2
Exhaust gas	Peltier cooled condenser
pH control	1-way control (2-way control optional)
pH Sensing	0-14
Polarographic DO sensing	Optional
DO control	Gas/liquid flow rate, stirrer speed (Optional)
BioVIS cell-density monitoring	Optional
Tandem off-gas analysis	Optional
Foam/Level detection	Optional
Control PC and software	WinISO control software (pre-installed)

Software

The H.E.L WinISO software runs every reactor with fully independent, automated control — advanced strategies from sensor feedback, time-based profiles or event detection, with real-time logging and .csv export.

Fully customizable, it controls the complete range of process parameters — temperature, stirrer speed, dissolved oxygen, pH, pressure, gas feeds & mix, liquid feed rates, biomass, off-gas analysis and foam & level detection.

Applications

Syngas fermentationCell-free biocatalysisCO₂ biomanufacturingSustainable aviation fuelAlternative proteinElevated pressure fermentation

Frequently asked questions

What is the BioXplorer 400P used for?

A bench-top, four-reactor high-pressure platform for gas-dependent bioprocesses — syngas fermentation, cell-free enzymatic processes, CO₂ biomanufacturing and Power-to-Liquid, through to SAF development.

Is the system customizable to my process?

Yes. Every BioXplorer 400P is configured to your requirements — probes, sensors, gas and liquid feeds are all specified around your application. Customisation to the customer’s process is central to H.E.L’s approach.

How many reactors and what working volume?

Four parallel, independently-controlled stainless-steel reactors with working volumes of 120–400 ml, fully autoclavable including sensors.

What is the maximum pressure?

Each reactor runs at up to 10 bar, with dual gas spargers per reactor to minimize hazardous-gas-mixture risk — ideal for pressure- and gas-dependent bioprocesses such as syngas fermentation.

What temperature and agitation does it offer?

0 to 135 °C with 0.1 °C resolution, with a choice of magnetic stirring (250–1500 rpm) or overhead agitation (20–2000 rpm).

What does the control software do?

The H.E.L WinISO software gives fully independent control of all bioprocess parameters across all four reactors: from automated stirring to complex sensor-driven gas and liquid additions, with real-time logging and .csv export.

What analytics and monitoring options are available?

pH (0–14) as standard, with optional dissolved-oxygen sensing, BioVIS cell-density monitoring and foam/level detection; a wide gas-calibration range supports air, O₂, N₂, H₂, CO, CO₂ and methane; optional Tandem off-gas analysis for OUR, CPR and RQ.

Does the system integrate PID control?

The BioXplorer platform utilizes precise proportional-integral-derivative (PID) control loops via WinISO software to manage crucial bioprocess parameters like temperature, gas flow and liquid feeds.