BioXplorer 5000 | Lab-scale bioreactor platform

The BioXplorer 5000 and 5000P single bioreactor systems are ideally suited to drive your scale-up efficiency. These systems are highly automated and highly customizable. The BioXplorer 5000P is the ideal choice for pressure applications up to 10 bar, ranging from syngas fermentation to processes driven by cell-free biocatalysis.

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Biotechnology Solutions

BioXplorer Specification Book

Features

The BioXplorer 5000 and 5000P single bioreactor systems are suitable for a wide range of applications including syngas fermentation and cell-free biocatalysis with:

Automated inventory monitoring
BioVIS optical probe to monitor cell density trends without taking samples
Choice of reactor sizes and types, including elevated pressure designs (up to 10 bar)
Fully automated control

Software

The H.E.L control software runs the BioXplorer 5000 system, enabling complex, fully automated experiments to run unattended.
This enables:

Full reactor control with unlimited step recipe planning. Plans can be changed online at any time without process interruption
The individual reactor temperature and agitation control
User-defined data-logging with customizable tabular and graphical displays
Export data functions and integration with in-house electronic lab notebooks and record-keeping systems
DoE and QbD package integration

More specifically, the software controls a wide range of system features, including:

Temperature
Gas feeds, flow and mix
pH
Dissolved oxygen
Redox
Stirrer speed
Torque (for viscosity indication)
Pressure
Liquid feed rates
Antifoam and level detection

Options

Gas Feeds

Choose from simple or more sophisticated gas flow control regimes
Bioreactor can be configured with single or multiple mass flow controllers
For microbial fermentations, automatic oxygen enrichment is possible
CO2 can be used for pH control
Ratios of several gases can be mixed and regulated with online feedback from off-gas analytics

Liquid Feeds

Choice of liquid feeds for standard and pressure applications
high pressure metering pumps enable exceptional pH and dosing control as well as a wide operating range

pH, Dissolved Oxygen and Redox

A wide range of probes are available to cater for different fermentation types, reactor sizes and applications
All probes are integrated into the WinISO software to allow automated monitoring and feedback loops

Off Gas Analysis

Tandem off-gas analysis units monitor and control a culture process continuously
This enables calculation of oxygen uptake rate (OUR); carbon dioxide production rate (CPR) and respiration quotient (RQ)

Cell Density and Biomass Monitoring

BioVIS enables the online monitoring of cell growth and biomass reducing requirements for online sampling
Viable cell density probes can also be integrated

Technical Literature

The following is a list of supporting Technical Literature.

The use of a H.E.L BioXplorer in fermentations of Cupriavidus necator H16 at elevated pressure using CO2 as the sole carbon source

Publications

The following are a list of some technical publications which highlight the use of the equipment.

Innovative high pressure/high temperature, multi-sensing bioreactors system for microbial risk assessment in underground hydrogen storage

Nicolò Santi Vasile, Ruggero Bellini, Ilaria Bassani, Arianna Vizzarro, Annalisa Abdel Azim, Christian Coti, Donatella Barbieri, Matteo Scapolo, Dario Viberti, Francesca Verga, Fabrizio Pirri, Barbara Menin

02-Jan-2024

https://www.sciencedirect.com/science/article/pii/S0360319923053934(Subscription or purchase maybe required for full access)